GABPA Monoclonal antibody

GABPA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A7E3

Cat no : 68645-1-Ig

Synonyms

E4TF1 60, E4TF1A, GA binding protein alpha chain, GABP subunit alpha, GABPA, NFT2, Transcription factor E4TF1 60

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Validation Data Gallery

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  WB analysis using 68645-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68645-1-Ig (GABPA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68645-1-Ig targets GABPA in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GABPA fusion protein Ag33705
• Full Name: GA binding protein transcription factor, alpha subunit 60kDa
• Calculated Molecular Weight: 454 aa, 51 kDa
• Observed Molecular Weight: 56-60 kDa
• GenBank Accession Number: BC035031
• Gene Symbol: GABPA
• Gene ID (NCBI): 2551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.