CoraLite® Plus 647 Anti-Mouse Foxp3 (3G3)

Foxp3 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3G3

Cat no : CL647-65089

Synonyms

forkhead box P3, Foxp3, JM2, scurfin, sf

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using CL647-65089

  1x10^6 mouse splenocytes were intracellularly stained with 0.125 ug CoraLite® Plus 647 Anti-Mouse Foxp3 (3G3) (CL647-65089, Clone:3G3) or 0.125 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21). Cells were co-stained with CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65089 targets Foxp3 in FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: forkhead box P3
• GenBank Accession Number: BC132333
• Gene Symbol: Foxp3
• Gene ID (NCBI): 20371
• RRID: AB_2920252
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011