FUT2 Polyclonal antibody

FUT2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20288-1-AP

Synonyms

Alpha(1,2)FT 2, B12QTL1, Fucosyltransferase 2, FUT2, SE, Se2, SEC2, Secretor factor, sej

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Validation Data Gallery

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  WB analysis using 20288-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20288-1-AP (FUT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, Calu-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20288-1-AP targets FUT2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FUT2 fusion protein Ag14047
• Full Name: fucosyltransferase 2 (secretor status included)
• Calculated Molecular Weight: 343 aa, 39 kDa
• Observed Molecular Weight: 35-39 kDa
• GenBank Accession Number: BC001899
• Gene Symbol: FUT2
• Gene ID (NCBI): 2524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fucosyltransferase 2 (FUT2) is a key enzyme that catalyzes the transfer of fucose to the terminal galactose of type 1 or type 2 disaccharide via α1,2-linkage. FUT2 gene codes for the alpha(1,2)fucosyltransferase responsible for the synthesis of the H antigen, which is the precursor of the ABO histo-blood group antigens in body fluids and on the intestinal mucosa (PMID: 19487333). FUT2 is highly expressed in lung adenocarcinoma (LUAD) and plays a vital role in the tumorigenesis of LUAD (PMID: 36552800). FUT2 has a calculated molecular mass of ~39 kDa, and the 70kDa band might be the glycosylated form.