FKBP9 Polyclonal antibody

FKBP9 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 18073-1-AP

Synonyms

63 kDa FK506 binding protein, 63 kDa FKBP, FK506 binding protein 9, FKBP 63, FKBP 9, FKBP60, FKBP63, FKBP9, PPIase, PPIase FKBP9, Rotamase

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 18073-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18073-1-AP (FKBP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 18073-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 18073-1-AP (FKBP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of L02 using 18073-1-AP

  Immunofluorescent analysis of L02 cells using 18073-1-AP (FKBP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, L02 cells
• Positive IF detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18073-1-AP targets FKBP9 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FKBP9 fusion protein Ag12715
• Full Name: FK506 binding protein 9, 63 kDa
• Calculated Molecular Weight: 570 aa, 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC101723
• Gene Symbol: FKBP9
• Gene ID (NCBI): 11328
• RRID: AB_2878490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP9 (FK506-binding protein 9, also referred to as FKBP60 or FKBP63) belongs to a family of immunophilins that are able to bind to FK506, an immunosuppressive drug. Several members of the FKBP family, including FKBP9, FKBP13, FKBP23 and FKBP65, localize to the endoplasmic reticulum (ER) as they contain the ER retention motif H/R/KDEL (PMID:32111229). In addition, the FKBP9 mutation was also reported to be associated with disease-free survival rates in pheochromocytoma or paraganglioma (PMID:28386672).