FILIP1L Polyclonal antibody

FILIP1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30134-1-AP

Synonyms

COL4A3BPIP, DOC 1, DOC1, FILIP1L, GIP90

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Validation Data Gallery

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  WB analysis using 30134-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30134-1-AP (FILIP1L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 30134-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 30134-1-AP (FILIP1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 30134-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 30134-1-AP (FILIP1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, mouse colon tissue
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30134-1-AP targets FILIP1L in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FILIP1L fusion protein Ag32655
• Full Name: filamin A interacting protein 1-like
• Calculated Molecular Weight: 893 aa, 102 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC027860
• Gene Symbol: FILIP1L
• Gene ID (NCBI): 11259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.