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CoraLite® Plus 488-conjugated FGL1 Monoclonal antibody

FGL1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B11C6

Cat no : CL488-67391

Synonyms

FGL1, fibrinogen like 1, Fibrinogen like protein 1, Hepassocin, HFREP 1, HFREP1, HP 041, LFIRE 1, LFIRE1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated FGL1 Monoclonal anti (CL488-67391)

    IF Staining of HepG2 using CL488-67391

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 FGL1 antibody (CL488-67391, Clone: 2B11C6 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67391 targets FGL1 in IF applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FGL1 fusion protein Ag8581
Full Name fibrinogen-like 1
Calculated Molecular Weight 312 aa, 36 kDa
GenBank Accession NumberBC007047
Gene Symbol FGL1
Gene ID (NCBI) 2267
RRIDAB_2919463
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibrinogen-like 1 (FGL1), also termed hepatocyte-derived fibrinogen-related protein 1 (HFREP1) or hepassocin, is a predominantly liver expressed protein that has been implicated as both a hepatoprotectant and a hepatocyte mitogen. FGL1 expression is decreased in hepatocellular carcinoma (HCC), and it may play a role in the development of hepatocellular carcinomas.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FGL1 antibody CL488-67391Download protocol
Standard Protocols
Click here to view our Standard Protocols