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FGL1 Polyclonal antibody

FGL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16000-1-AP

Synonyms

FGL1, fibrinogen like 1, Fibrinogen like protein 1, Hepassocin, HFREP 1, HFREP1, HP 041, HPS, LFIRE 1, LFIRE1



Tested Applications

Positive WB detected inhuman placenta tissue, human liver tissue, HepG2 cells, mouse thymus tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human hepatocirrhosis tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16000-1-AP targets FGL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGL1 fusion protein Ag8581
Full Name fibrinogen-like 1
Calculated Molecular Weight 312 aa, 36 kDa
Observed Molecular Weight 34 kDa, 68 kDa
GenBank Accession NumberBC007047
Gene Symbol FGL1
Gene ID (NCBI) 2267
RRIDAB_2103936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGL1 is a 68 kDa protein that comprises two disulfide linked 34 kDa homodimers. FGL1 is a predominantly liver expressed protein that has been implicated as both a hepatoprotectant and a hepatocyte mitogen. FGL1 expression is decreased in hepatocellular carcinoma (HCC), and it may play a role in the development of hepatocellular carcinomas.

Protocols

Product Specific Protocols
WB protocol for FGL1 antibody 16000-1-APDownload protocol
IHC protocol for FGL1 antibody 16000-1-APDownload protocol
IP protocol for FGL1 antibody 16000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Transl Res

Role of placental fibrinogen-like protein 1 in gestational diabetes.

Authors - Lin Kang
mouseIHC

Front Immunol

Fibrinogen-like protein 1 promotes liver-resident memory T-cell exhaustion in hepatocellular carcinoma

Authors - Changjie Yang
mouseWB

Diabetologia

PMID: 27221093

Authors - Hung-Tsung Wu
  • KD Validated
mouseWB,IHC

J Nanobiotechnology

Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles.

Authors - Chunai Gong
humanWB

Int J Mol Sci

Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells.

Authors - Yeonghoon Son
human,mouseWB

J Clin Endocrinol Metab

The Hepatic Protection Effects of Hepassocin in Hyperglycemic Crisis.

Authors - Horng-Yih Ou