FEM1B Polyclonal antibody

FEM1B Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11030-1-AP

Synonyms

F1A alpha, F1AA, fem 1 homolog b (C. elegans), FEM1 beta, FEM1B, FIAA, KIAA0396, Protein fem 1 homolog B

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Validation Data Gallery

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  WB analysis using 11030-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11030-1-AP (FEM1B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11030-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11030-1-AP (FEM1B antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse testis tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11030-1-AP targets FEM1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FEM1B fusion protein Ag1497
• Full Name: fem-1 homolog b (C. elegans)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC010122
• Gene Symbol: FEM1B
• Gene ID (NCBI): 10116
• RRID: AB_2102637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FEM1B, also named F1AA, and KIAA0396 is a 627 amino acid protein, which belongs to the fem-1 family. FEM1B is highly expressed in the testis and weakly expressed in other tissues. FEM1B localizes both in the nucleus and cytoplasm. In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment. FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. FEM1B is also involved in glucose homeostasis in pancreatic islets. FEM1B functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.