FCHO1 Polyclonal antibody

FCHO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26767-1-AP

Synonyms

FCH domain only 1, FCH domain only protein 1, FCHO1, KIAA0290

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Validation Data Gallery

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  WB analysis of Raji using 26767-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 26767-1-AP (FCHO1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 26767-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26767-1-AP (FCHO1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26767-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26767-1-AP (FCHO1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26767-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26767-1-AP (FCHO1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Raji cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26767-1-AP is FCHO1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FCHO1 fusion protein Ag25291
• Full Name: FCH domain only 1
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC028021
• Gene Symbol: FCHO1
• Gene ID (NCBI): 23149
• RRID: AB_2880626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FCHO1 is composed of 889 amino acid residues and contains an N-terminal FES-CIP4 homology (FCH) domain and an evolutionarily conserved C-terminal FCHO homology (FOH) domain. FCHO1 functions in an early step of clathrin-mediated endocytosis. FCHO1 is required for plasma membrane clathrin-coated vesicle (CCV) budding and marked sites of CCV formation (PMID: 20448150).