FARS2 Polyclonal antibody

FARS2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16436-1-AP

Synonyms

FARS1, FARS2, HSPC320, Phenylalanine tRNA ligase, PheRS

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Validation Data Gallery

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  WB analysis using 16436-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16436-1-AP (FARS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

16436-1-AP targets FARS2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FARS2 fusion protein Ag9750
• Full Name: phenylalanyl-tRNA synthetase 2, mitochondrial
• Calculated Molecular Weight: 451 aa, 52 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC021112
• Gene Symbol: FARS2
• Gene ID (NCBI): 10667
• RRID: AB_2102499
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FARS2, a nuclear gene located on chromosome 6 (6p25.1), encodes the mitochondrial phenylalanyl-tRNA synthetase (mtPheRS), which transfers phenylalanine (Phe) to its cognate tRNA in mitochondria (PMID: 32774346).

Publications

Species	Application	Title
human	WB	Mol Genet Metab; Clinical and molecular characterization of novel FARS2 variants causing neonatal mitochondrial disease; Authors - Wenqian Chen
human	WB	Mol Genet Metab; New insights into the phenotype of FARS2 deficiency.; Authors - Elise Vantroys
human	WB	Biochim Biophys Acta; Mutation of the human mitochondrial phenylalanine-tRNA synthetase causes infantile-onset epilepsy and cytochrome c oxidase deficiency.; Authors - Abdulraheem Almalki
human	WB	Eur J Hum Genet; Novel FARS2 variants in patients with early onset encephalopathy with or without epilepsy associated with long survival.; Authors - Giulia Barcia
human,rat,mouse	WB	Nat Commun; Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.; Authors - Qian Zhou