FAM71E2 Polyclonal antibody

FAM71E2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20904-1-AP

Synonyms

C19orf16, FAM71E2, Protein FAM71E2

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Validation Data Gallery

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  WB analysis of human brain using 20904-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20904-1-AP (FAM71E2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20904-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20904-1-AP (FAM71E2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 20904-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20904-1-AP (FAM71E2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 20904-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20904-1-AP (FAM71E2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 20904-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20904-1-AP (FAM71E2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20904-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20904-1-AP (FAM71E2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, HepG2 cells
• Positive IHC detected in: human brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20904-1-AP is FAM71E2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM71E2 fusion protein Ag15027
• Full Name: family with sequence similarity 71, member E2
• Calculated Molecular Weight: 302 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC031875
• Gene Symbol: FAM71E2
• Gene ID (NCBI): 284418
• RRID: AB_11183765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.