FAM164C Polyclonal antibody

FAM164C Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20520-1-AP

Synonyms

C14orf140, FAM164C, Protein FAM164C

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Validation Data Gallery

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  WB analysis of HeLa using 20520-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20520-1-AP (FAM164C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 20520-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 20520-1-AP (FAM164C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20520-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20520-1-AP (FAM164C Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human testis using 20520-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 20520-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 20520-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20520-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of C6 using 20520-1-AP

  Immunofluorescent analysis of C6 cells using 20520-1-AP (FAM164C antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, C6 cells
• Positive IHC detected in: human kidney tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20520-1-AP is FAM164C Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM164C fusion protein Ag14359
• Full Name: family with sequence similarity 164, member C
• Calculated Molecular Weight: 275 aa, 31 kDa
• Observed Molecular Weight: 31-36 kDa
• GenBank Accession Number: BC004259
• Gene Symbol: FAM164C
• Gene ID (NCBI): 79696
• RRID: AB_2878697
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM164C, also named as ZC2HC1C or C14orf140, is a 456 amino acid protein, which contains one C2HC-type zinc finger and belongs to the ZC2HC1 family. FAM164C exists as two isoforms. The isoform1 is a 51kDa protein ad isoform2 is 31kDa.