CoraLite®555-conjugated FAH Monoclonal antibody

FAH Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

IF

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2F8E11

Cat no : CL555-67986

Synonyms

Beta diketonase, FAA, FAH, Fumarylacetoacetase, Fumarylacetoacetate hydrolase

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Validation Data Gallery

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  IF Staining of HepG2 using CL555-67986

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®555 FAH antibody (CL555-67986, Clone: 2F8E11 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67986 targets FAH in IF applications and shows reactivity with Human, pig, rat, mouse samples.

• Tested Reactivity: Human, pig, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FAH fusion protein Ag6704
• Full Name: fumarylacetoacetate hydrolase (fumarylacetoacetase)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC002527
• Gene Symbol: FAH
• Gene ID (NCBI): 2184
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAH is also named as FAA(fumarylacetoacetase), the last enzyme in tyrosine degradation, functionally important in the liver and kidney(PMID:8473520). FAH is a metabolic enzyme catalyzing the last step of tyrosine and phenylalanine catabolism: the hydrolysis of fumarylacetoacetate into acetoacetate and fumarate. In humans, deficiency of this activity is associated with the metabolic disease hereditary tyrosinaemia type I, which is also known as hepatorenal tyrosinaemia(PMID:17768357).