EXOSC7 Monoclonal antibody

EXOSC7 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A4C6

Cat no : 68456-1-Ig

Synonyms

EAP1, EXOSC7, exosome component 7, FLJ26543, hRrp42p, KIAA0116, p8, RRP42, Rrp42p

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Validation Data Gallery

• 

  WB analysis using 68456-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68456-1-Ig (EXOSC7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68456-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68456-1-Ig (EXOSC7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Saos-2 cells, HSC-T6 cells, Caco-2 cells, LNCaP cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, 4TA cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68456-1-Ig targets EXOSC7 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EXOSC7 fusion protein Ag18322
• Full Name: exosome component 7
• Calculated Molecular Weight: 291 aa, 32 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC012831
• Gene Symbol: EXOSC7
• Gene ID (NCBI): 23016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. EXOSC7 also named as RRP42 or EAP1 is a 291 amino acid protein, which belongs to the RNase PH family. EXOSC7 is one of the six RNase-PH domain subunits of the exosome and required for the processing of the 7S pre-RNA.