EVI1 Polyclonal antibody

EVI1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23201-1-AP

Synonyms

AML1 EVI 1, EVI 1, EVI1, MDS1 EVI1, MECOM, PRDM3

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Validation Data Gallery

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  WB analysis of SKOV-3 using 23201-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 23201-1-AP (EVI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 23201-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 23201-1-AP (EVI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid cancer using 23201-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 23201-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 23201-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 23201-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SKOV-3 using 23201-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SKOV-3 cells using 23201-1-AP (EVI1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SKOV-3 cells
• Positive IHC detected in: human urothelial carcinoma tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

23201-1-AP targets EVI1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EVI1 fusion protein Ag19603
• Full Name: ecotropic viral integration site 1
• Calculated Molecular Weight: 1239 aa, 139 kDa
• Observed Molecular Weight: 118 kDa
• GenBank Accession Number: BC130520
• Gene Symbol: EVI1
• Gene ID (NCBI): 2122
• RRID: AB_2879230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Sci Adv; Predicting master transcription factors from pan-cancer expression data.; Authors - Jessica Reddy
human	CoIP	Oncol Rep; Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis; Authors - Zhitao Chen