CoraLite® Plus 647-conjugated ERP29 Monoclonal antibody

ERP29 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1H8B2

Cat no : CL647-67675

Synonyms

C12orf8, ERp28, ERP29, ERp31, PDI DB, PDIA9

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Validation Data Gallery

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  IF Staining of human kidney using CL647-67675

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 647 ERP29 antibody (CL647-67675, Clone: 1H8B2 ) at dilution of 1:200.

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  IF Staining of human kidney using CL647-67675

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 647 ERP29 antibody (CL647-67675, Clone: 1H8B2 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human kidney tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67675 targets ERP29 in IF applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERP29 fusion protein Ag30262
• Full Name: endoplasmic reticulum protein 29
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC101495
• Gene Symbol: ERP29
• Gene ID (NCBI): 10961
• RRID: AB_2920298
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endoplasmic reticulum resident protein 29 (ERP29) is a 29-kDa endoplasmic reticulum (ER) luminal protein. Structurally ERp29 is related to protein disulfide isomerases (PDI). However, it lacks the thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase (PMID: 15500441). ERP29 plays an important role in the processing of secretory proteins within the (ER), possibly by participating in the folding of proteins in the ER. The secretion of thyroglobulin (TG) was found to be strongly regulated by ERP29 (PMID: 16380091).