ERGIC3 Monoclonal antibody

ERGIC3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B9F11

Cat no : 68398-1-Ig

Synonyms

C20orf47, CGI 54, dJ477O4.2, ERGIC and golgi 3, ERGIC3, Erv46, NY BR 84, PRO0989, SDBCAG84

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Validation Data Gallery

• 

  WB analysis using 68398-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68398-1-Ig (ERGIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NCI-H1299 cells, Calu-3 cells, U2OS cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68398-1-Ig targets ERGIC3 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERGIC3 fusion protein Ag9190
• Full Name: ERGIC and golgi 3
• Calculated Molecular Weight: 383 aa, 43 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC009765
• Gene Symbol: ERGIC3
• Gene ID (NCBI): 51614
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERGIC3 (Endoplasmic reticulum-Golgi intermediate compartment protein 3 ) is located in the cis face of the Golgi apparatus and vesicular tubular structures between the transitional endoplasmic reticulum (ER) and cis-Golgi. ERGIC3 significantly affects cell growth and causes ER stress-induced cell death, and is involved in the invasion and metastasis in hepatocellular carcinomas (HCC). (PMID: 26177443, PMID: 31142615)