ERGIC3 Polyclonal antibody

ERGIC3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16029-1-AP

Synonyms

C20orf47, CGI 54, ERGIC and golgi 3, ERGIC3, Erv46, NY BR 84, PRO0989, SDBCAG84

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Validation Data Gallery

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  WB analysis using 16029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16029-1-AP (ERGIC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 16029-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16029-1-AP (ERGIC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16029-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16029-1-AP (ERGIC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16029-1-AP

  Immunofluorescent analysis of HepG2 cells using 16029-1-AP (ERGIC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, MCF-7 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16029-1-AP targets ERGIC3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERGIC3 fusion protein Ag8893
• Full Name: ERGIC and golgi 3
• Calculated Molecular Weight: 383 aa, 43 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC009765
• Gene Symbol: ERGIC3
• Gene ID (NCBI): 51614
• RRID: AB_2098446
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ERGIC3 protein was also over-expressed in lung cancer tissues and cultured cells, and expression of ERGIC3 was correlated with the differentiated degree and histological type of lung cancer (PMID: 23374247). The up-regulation of ERGIC3 could promote cellular migration and proliferation in vitro. ERGIC3 may play an active role in the development and progression of lung cancer (PMID: 23374247). The MW of this protein is 50 kDa, and this antibody specially recognises the 50 kDa protein.

Publications

Species	Application	Title
human	WB	J Biol Chem; Identification of TRAMs as Sphingolipid-Binding Proteins Using a Photoactivatable and Clickable Short Chain Ceramide Analog.; Authors - Yaqin Deng