CD39/ENTPD1 Monoclonal antibody

CD39/ENTPD1 Monoclonal Antibody for IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D2H6

Cat no : 60323-1-Ig

Synonyms

ATPDase, CD39, CD39/ENTPD1, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, e-NTPDase, NTPDase 1

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Validation Data Gallery

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  WB analysis using 60323-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 60323-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 60323-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 60323-1-Ig

  Immunofluorescent analysis of HeLa cells using 60323-1-Ig (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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  FC experiment of Jurkat using 60323-1-Ig

  1X10^6 Jurkat cells were stained with 0.2ug ENTPD1 antibody (60323-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60323-1-Ig targets CD39/ENTPD1 in IF, FC, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD39/ENTPD1 fusion protein Ag6165
• Full Name: ectonucleoside triphosphate diphosphohydrolase 1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC047664
• Gene Symbol: ENTPD1
• Gene ID (NCBI): 953
• RRID: AB_2881434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing. The soluble isoform of CD39(38-478 amino acid) is located in the extracellular part and 60323-1-Ig can recognize the soluble form of CD39 (38-478aa) molecule.