ENOPH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ENOPH1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11763-1-AP

Synonyms

E1, enolase phosphatase 1, Enolase phosphatase E1, ENOPH1, MASA, MASA homolog, MST145

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Validation Data Gallery

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  WB analysis using 11763-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11763-1-AP (ENOPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 11763-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11763-1-AP (ENOPH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

11763-1-AP targets ENOPH1 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENOPH1 fusion protein Ag2360
• Full Name: enolase-phosphatase 1
• Calculated Molecular Weight: 13 kDa / 29 kDa
• Observed Molecular Weight: 29-35 kDa
• GenBank Accession Number: BC001317
• Gene Symbol: ENOPH1
• Gene ID (NCBI): 58478
• RRID: AB_10794441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENOPH1, also named as MASA and MSTP145, belongs to the HAD-like hydrolase superfamily and MasA/MtnC family. ENOPH1 is a newly identified enzyme involved in L-methionine biosynthesis, which is associated with anxiety and depression. Studies have shown that ENOPH1 plays a crucial role in promoting the proliferation and migration of glioma cells (PMID: 32654229). ENOPH1 has 2 isoforms with the molecular mass of 13 and 29 kDa.

Publications

Species	Application	Title
human	WB,IF	Front Mol Neurosci; Cerebral Microvascular Endothelial Cell Apoptosis after Ischemia: Role of Enolase-Phosphatase 1 Activation and Aci-Reductone Dioxygenase 1 Translocation.; Authors - Yuan Zhang ; KD Validated
human	WB	Oncol Rep; Enolase-phosphatase 1 as a novel potential malignant glioma indicator promotes cell proliferation and migration.; Authors - Li Su ; KD Validated