CoraLite® Plus 488-conjugated ENO3 Monoclonal antibody

ENO3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C12G3

Cat no : CL488-68147

Synonyms

Beta enolase, ENO3, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

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Validation Data Gallery

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  IF Staining of HeLa using CL488-68147

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ENO3 antibody (CL488-68147, Clone: 2C12G3 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68147 targets ENO3 in IF applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ENO3 fusion protein Ag9642
• Full Name: enolase 3 (beta, muscle)
• Calculated Molecular Weight: 434 aa, 47 kDa
• Observed Molecular Weight: 42-47 kDa
• GenBank Accession Number: BC017249
• Gene Symbol: ENO3
• Gene ID (NCBI): 2027
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Enolase 3 (ENO3) encodes the β-subunit of enolase, which is distributed in various tissues, including liver, lung, skeletal, and heart. ENO3 plays an important role in both glycogen and cholesterol metabolism. It has been demonstrated that ENO3 deficiency may lead to metabolic myopathies, and ENO3 speeds up hepatic cholesterol ester cumulation induced via the mediation of cholesteryl ester generation.Apart from these, ENO3 has also been involved in various tumors, though the results are contradictory. For example, ENO3 is up-regulated in STK11 mutant pulmonary carcinoma and colorectal carcinoma, while down-regulated in pancreatic cancer and hepatocellular carcinomas. ENO3 has 3 isoforms with the molecular mass of 42-47 kDa. (PMID: 33987359, 35004693)