CoraLite® Plus 488-conjugated EMILIN1 Monoclonal antibody

EMILIN1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A5C8

Cat no : CL488-60047

Synonyms

DKFZp586M121, EMI, EMILIN, EMILIN 1, EMILIN1, gp115

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Validation Data Gallery

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  IF Staining of human ovary tumor using CL488-60047

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 EMILIN1 antibody (CL488-60047, Clone: 2A5C8 ) at dilution of 1:200.

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  IF Staining of human ovary tumor using CL488-60047

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 EMILIN1 antibody (CL488-60047, Clone: 2A5C8 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human ovary tumor tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60047 targets EMILIN1 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EMILIN1 fusion protein Ag1016
• Full Name: elastin microfibril interfacer 1
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC007530
• Gene Symbol: EMILIN1
• Gene ID (NCBI): 11117
• RRID: AB_2919227
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).