EMCN Polyclonal antibody

EMCN Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11828-1-AP

Synonyms

EMCN, EMCN2, endomucin, Endomucin 2, Gastric cancer antigen Ga34, MUC 14, MUC14, Mucin 14

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Validation Data Gallery

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  WB analysis using 11828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11828-1-AP (EMCN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 11828-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11828-1-AP (EMCN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11828-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11828-1-AP (EMCN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse kidney tissue, mouse lung tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11828-1-AP targets EMCN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EMCN fusion protein Ag2396
• Full Name: endomucin
• Calculated Molecular Weight: 19 kDa, 27 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC017781
• Gene Symbol: EMCN
• Gene ID (NCBI): 51705
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endomucin (EMCN), also known as Endomucin 2 or Mucin 14, is a type I integral membrane glycoprotein that is endothelial-specific. EMCN is expressed on the surface of capillaries and venous and is highly expressed in highly vascularized tissues such as heart, kidney and lung (PMID: 29215001). Endomucin plays an important role in cell interaction, molecular cell signaling, angiogenesis and cell migration (PMID: 33532708). Since it is glycosylated, the apparent molecular weight of EMCN could be variable, ranging from 80 kDa to 120 kDa.