ELP2 Polyclonal antibody

ELP2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 13793-1-AP

Synonyms

Elongator complex protein 2, ELP2, SHINC 2, STAT3 interacting protein 1, STATIP1, StIP, StIP1

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Validation Data Gallery

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  IHC staining of human colon cancer using 13793-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13793-1-AP (ELP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13793-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13793-1-AP (ELP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13793-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13793-1-AP (ELP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13793-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13793-1-AP (ELP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13793-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13793-1-AP (ELP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 13793-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13793-1-AP (ELP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 13793-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13793-1-AP (ELP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13793-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13793-1-AP (ELP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human stomach cancer tissue, mouse liver tissue, human colon cancer tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13793-1-AP targets ELP2 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ELP2 fusion protein Ag4581
• Full Name: elongation protein 2 homolog (S. cerevisiae)
• Calculated Molecular Weight: 826 aa, 93 kDa
• GenBank Accession Number: BC032553
• Gene Symbol: ELP2
• Gene ID (NCBI): 55250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELP2 is a component of the elongator complex which is required for multiple tRNA modifications. The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.