CoraLite® Plus 647-conjugated EIF3L Monoclonal antibody

EIF3L Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1B11A10

Cat no : CL647-67719

Synonyms

EIF3EIP, EIF3L, EIF3S11, EIF3S6IP, HSPC021, HSPC025, MSTP005

---

Validation Data Gallery

• 

  FC experiment of HeLa using CL647-67719

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human EIF3L (CL647-67719, Clone:1B11A10) (red), or 0.8 ug CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67719 targets EIF3L in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: EIF3L fusion protein Ag26680
• Full Name: eukaryotic translation initiation factor 3, subunit L
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC007510
• Gene Symbol: EIF3L
• Gene ID (NCBI): 51386
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.