EIF2S2 Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF2S2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 10227-1-AP

Synonyms

eIF 2 beta, EIF2, EIF2B, EIF2beta, EIF2S2

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Validation Data Gallery

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  WB analysis of mouse liver using 10227-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10227-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10227-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10227-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10227-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10227-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10227-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10227-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10227-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10227-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10227-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10227-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10227-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10227-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10227-1-AP (EIF2S2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10227-1-AP

  IP result of anti-EIF2S2 (IP:10227-1-AP, 4ug; Detection:10227-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human testis using 10227-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10227-1-AP (EIF2S2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 10227-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10227-1-AP (EIF2S2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10227-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10227-1-AP (EIF2S2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10227-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10227-1-AP (EIF2S2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 10227-1-AP

  Immunofluorescent analysis of HepG2 cells, using EIF2S2 antibody 10227-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of L02 using 10227-1-AP

  Immunofluorescent analysis of L02 cells using 10227-1-AP (EIF2S2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse testis tissue, mouse liver tissue, L02 cells, HeLa cells, PC-3 cells, HepG2 cells, rat liver tissue, Jurkat cells, NIH/3T3 cells, C6 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human testis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below

Product Information

10227-1-AP targets EIF2S2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF2S2 fusion protein Ag0269
• Full Name: eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC000461
• Gene Symbol: EIF2S2
• Gene ID (NCBI): 8894
• RRID: AB_2262043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation initiation factor 2 (eIF2) is composed of three subunits, eIF2 alpha, eIF2 beta (EIF2S2), and eIF2 gamma, which are present in equal molar amounts. eIF2 beta plays a central role in the maintenance of what is generally considered a rate-limiting step in mRNA translation. In the early steps of protein synthesis, eIF2 beta binds GTP and Met-tRNA and transfers Met-tRNA to the 40S ribosomal subunit. At the end of the initiation process, GTP bound to eIF2 beta is hydrolyzed to GDP and the eIF2/GDP complex is released from the ribosome. The exchange of GDP bound to eIF2 beta for GTP is a prerequisite to binding Met-tRNA and is mediated by eIF2 beta, which recycles the eIF2 complex for another round of initiation.

Publications

Species	Application	Title
human	WB	Cancer Res; Comparative oncogenomics identifies PSMB4 and SHMT2 as potential cancer driver genes.; Authors - Genee Y Lee
human	WB	Front Mol Biosci; Comprehensive Characterization of Ageing-Relevant Subtypes Associated With Different Tumorigenesis and Tumor Microenvironment in Prostate Cancer.; Authors - Liang Huang ; KD Validated
mouse	WB	Neurogenetics; Genetic ablation of ataxin-2 increases several global translation factors in their transcript abundance but decreases translation rate.; Authors - M Fittschen
	WB	Fly (Austin); Native purification of protein and RNA-protein complexes using a novel affinity procedure.; Authors - Matthias Kroiss
human	WB	Neurobiol Dis; Dissecting the roles of EIF4G homologs reveals DAP5 as a modifier of CGG repeat-associated toxicity in a Drosophila model of FXTAS; Authors - Indranil Malik ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Leos (Verified Customer) (08-05-2022)

eIF2S2 is good for IP and WB, but eIF2S3 is only for WB

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

EIF2S2 Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in HEK293T (Cat no:10227-1-AP)