EFTUD1 Polyclonal antibody

EFTUD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24729-1-AP

Synonyms

EFTUD1, FAM42A, HsT19294, Protein FAM42A, RIA1

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Validation Data Gallery

• 

  WB analysis of mouse brain using 24729-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 24729-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 24729-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24729-1-AP (EFTUD1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 24729-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24729-1-AP (EFTUD1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, fetal human brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24729-1-AP is EFTUD1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EFTUD1 fusion protein Ag20371
• Full Name: elongation factor Tu GTP binding domain containing 1
• Calculated Molecular Weight: 1120 aa, 125 kDa
• Observed Molecular Weight: 125-135 kDa
• GenBank Accession Number: BC156743
• Gene Symbol: EFTUD1
• Gene ID (NCBI): 79631
• RRID: AB_2879693
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.