DYNLL2 Polyclonal antibody

DYNLL2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 16811-1-AP

Synonyms

8 kDa dynein light chain b, Dlc2, DLC8b, DNCL1B, Dynein light chain LC8 type 2, DYNLL2

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Validation Data Gallery

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  WB analysis using 16811-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16811-1-AP (DYNLL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16811-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16811-1-AP (DYNLL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 16811-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16811-1-AP (DYNLL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, human heart tissue, mouse brain tissue, rat brain tisssue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

16811-1-AP targets DYNLL2 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYNLL2 fusion protein Ag10345
• Full Name: dynein, light chain, LC8-type 2
• Calculated Molecular Weight: 89 aa, 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC010744
• Gene Symbol: DYNLL2
• Gene ID (NCBI): 140735
• RRID: AB_2093667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
human	WB	Cell Cycle; Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis.; Authors - Ankur A Gholkar
human	IHC	Front Oncol; TRIM68, PIKFYVE, and DYNLL2: The Possible Novel Autophagy- and Immunity-Associated Gene Biomarkers for Osteosarcoma Prognosis.; Authors - Jie Jiang
human		Biochim Biophys Acta Mol Cell Res; Interactions between two regulatory proteins of microtubule dynamics, HDAC6, TPPP/p25, and the hub protein, DYNLL/LC8.; Authors - Judit Oláh
human	WB,CoIP	Sci Rep; The interaction between LC8 and LCA5 reveals a novel oligomerization function of LC8 in the ciliary-centrosome system; Authors - Tamás Szaniszló