CoraLite® Plus 488-conjugated DVL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

DVL2 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12037

Synonyms

Dishevelled 2, DSH homolog 2, DVL2

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-12037

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 DVL2 antibody (CL488-12037) at dilution of 1:400.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12037 targets DVL2 in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DVL2 fusion protein Ag2666
• Full Name: dishevelled, dsh homolog 2 (Drosophila)
• Calculated Molecular Weight: 736 aa, 79 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC014844
• Gene Symbol: DVL2
• Gene ID (NCBI): 1856
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DVL2 is member of the dishevelled (dsh) protein family. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein in a variety of cell lines and species. DVL2 participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. It promotes internalization and degradation of frizzled proteins upon Wnt signaling. It plays a role both in canonical and non-canonical Wnt signaling. It also Plays a role in the signal transduction pathways mediated by multiple Wnt genes.