DSN1 Polyclonal antibody

DSN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17742-1-AP

Synonyms

C20orf172, DSN1, MIS13

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Validation Data Gallery

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  WB analysis of Jurkat using 17742-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17742-1-AP (DSN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17742-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17742-1-AP (DSN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17742-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17742-1-AP (DSN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

17742-1-AP targets DSN1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DSN1 fusion protein Ag11988
• Full Name: DSN1, MIND kinetochore complex component, homolog (S. cerevisiae)
• Calculated Molecular Weight: 340 aa, 38 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC026011
• Gene Symbol: DSN1
• Gene ID (NCBI): 79980
• RRID: AB_2878431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DSN1, also named as C20orf172 and MIS13, is required to shape an interface between the Mis12 complex and Blinkin, the counterpart of Spc105. DSN1 is part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. DSN1 has four isoforms with MW 27-33 kDa and 38-40 kDa.

Publications

Species	Application	Title
human	IF	Nat Commun; Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.; Authors - Haining Zhou
human	WB	J Exp Clin Cancer Res; Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma.; Authors - Huakai Wang