DOK7 Polyclonal antibody

DOK7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22697-1-AP

Synonyms

C4orf25, CMS1B, docking protein 7, Dok 7, DOK7, Protein Dok 7

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Validation Data Gallery

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  WB analysis of HEK-293 using 22697-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22697-1-AP (DOK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22697-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22697-1-AP (DOK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22697-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22697-1-AP (DOK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 22697-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22697-1-AP (DOK7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22697-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22697-1-AP (DOK7 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, HEK-293 cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22697-1-AP is DOK7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DOK7 fusion protein Ag18543
• Full Name: docking protein 7
• Calculated Molecular Weight: 504 aa, 53 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC131544
• Gene Symbol: DOK7
• Gene ID (NCBI): 285489
• RRID: AB_2879152
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOK7, also named as C4orf25, is a MuSK-interacting cytoplasmic protein. DOK7 is essential for neuromuscular synaptogenesis through its interaction with MuSK. It plays an essential role in neuromuscular synaptogenesis. DOK7 acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. DOK7 has some isoforms with MW 53 kDa, 37 kDa, 28 kDa and 64 kDa.