DOCK6 Polyclonal antibody

DOCK6 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25087-1-AP

Synonyms

dedicator of cytokinesis 6, DOCK6, KIAA1395, ZIR1

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Validation Data Gallery

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  WB analysis using 25087-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25087-1-AP (DOCK6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 25087-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25087-1-AP (DOCK6 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 25087-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25087-1-AP (DOCK6 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 25087-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25087-1-AP (DOCK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25087-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25087-1-AP (DOCK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells
• Positive IHC detected in: human colon tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25087-1-AP is DOCK6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DOCK6 fusion protein Ag18901
• Full Name: dedicator of cytokinesis 6
• Calculated Molecular Weight: 2047 aa, 230 kDa
• Observed Molecular Weight: 230 kDa
• GenBank Accession Number: BC146786
• Gene Symbol: DOCK6
• Gene ID (NCBI): 57572
• RRID: AB_2879889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.; Authors - Li Wang
human	WB	BMC Med Genomics; Attenuated clinical and osteoclastic phenotypes of Paget's disease of bone linked to the p.Pro392Leu/SQSTM1 mutation by a rare variant in the DOCK6 gene.; Authors - Mariam Dessay