DNER Polyclonal antibody

DNER Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24362-1-AP

Synonyms

bet, DNER, UNQ26

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Validation Data Gallery

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  WB analysis using 24362-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24362-1-AP (DNER antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 24362-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24362-1-AP (DNER antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 24362-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24362-1-AP (DNER antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24362-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24362-1-AP (DNER antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24362-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24362-1-AP (DNER antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, pig cerebellum tissue
• Positive IHC detected in: mouse cerebellum tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24362-1-AP targets DNER in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNER fusion protein Ag19955
• Full Name: delta/notch-like EGF repeat containing
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: BC035009
• Gene Symbol: DNER
• Gene ID (NCBI): 92737
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Delta and Notch-like epidermal growth factor-related receptor (DNER) is a type I transmembrane protein that is specifically expressed in the developing and mature central nervous system (PMID: 11950833). It contains ten extracellular EGF-like repeats that are closely related to those of the developmentally important receptor Notch and its ligand Delta. DNER functions as a Notch ligand and mediates signaling through neuron-glia interactions (PMID: 15965470).