DNAJC21 Monoclonal antibody

DNAJC21 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2F8A9

Cat no : 68343-1-Ig

Synonyms

DNAJA5, DNAJC21, GS3, JJJ1, Protein GS3

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Validation Data Gallery

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  WB analysis using 68343-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68343-1-Ig (DNAJC21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IF Staining of U2OS using 68343-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DNAJC21 antibody (68343-1-Ig, Clone: 2F8A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IF detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68343-1-Ig targets DNAJC21 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DNAJC21 fusion protein Ag20003
• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 21
• Calculated Molecular Weight: 531 aa, 58 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC107577
• Gene Symbol: DNAJC21
• Gene ID (NCBI): 134218
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJC21 is a ubiquitously expressed DnaJ (heat shock protein 40) chaperone protein. Recently DNAJC21 was reported to be implicated in rRNA biogenesis. Loss of DNAJC21 induced abnormal cell morphology and inhibited cell growth. Mutations in DNAJC21 cause bone marrow failure syndrome. (27346687)