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DNAJC12 Polyclonal antibody

DNAJC12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12338-1-AP

Synonyms

DNAJC12, J domain containing protein 1, JDP1



Tested Applications

Positive WB detected inA549 cells, BxPC-3 cells, HEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12338-1-AP targets DNAJC12 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC12 fusion protein Ag2989
Full Name DnaJ (Hsp40) homolog, subfamily C, member 12
Calculated Molecular Weight 198 aa, 23 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC017018
Gene Symbol DNAJC12
Gene ID (NCBI) 56521
RRIDAB_2246188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DNAJC12 antibody 12338-1-APDownload protocol
IHC protocol for DNAJC12 antibody 12338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Rep Med

Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application

Authors - Xiaoxue Ren
humanWB

Front Oncol

DNAJC12 as a Mediator Between ESR1 and ERBB4 in Breast Carcinoma Cells.

Authors - Mianjie Lin
humanWB,IF

Cell Stress Chaperones

The co-chaperone DNAJC12 binds to Hsc70 and is upregulated by endoplasmic reticulum stress.

Authors - Choi Jin J
humanWB

Ann Surg Oncol

Increased Expression of DNAJC12 is Associated with Aggressive Phenotype of Gastric Cancer.

Authors - Yasuo Uno
  • KD Validated