CoraLite® Plus 647-conjugated DLL3 Polyclonal antibody

DLL3 Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-25535

Synonyms

delta like 3 (Drosophila), Delta like protein 3, Delta3, DLL3, Drosophila Delta homolog 3, SCDO1

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Validation Data Gallery

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  IF Staining of mouse brain using CL647-25535

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 DLL3 antibody (CL647-25535) at dilution of 1:400.

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  IF Staining of mouse brain using CL647-25535

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 DLL3 antibody (CL647-25535) at dilution of 1:400.

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  FC experiment of U-87 MG using CL647-25535

  1X10^6 U-87 MG cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human DLL3 (CL647-25535) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: mouse brain tissue
• Positive FC detected in: U-87 MG cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-25535 targets DLL3 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DLL3 fusion protein Ag21965
• Full Name: delta-like 3 (Drosophila)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC000218
• Gene Symbol: DLL3
• Gene ID (NCBI): 10683
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Delta-Notch pathway is an evolutionarily conserved signaling pathway which controls a broad range of developmental processes including cell fate determination, terminal differentiation and proliferation (PMID: 22353464). In mammals, four Notch receptors (NOTCH1-4) and five activating canonical ligands (JAGGED1, JAGGED2, DLL1, DLL3 and DLL4) have been described (PMID: 22353464). DLL3 is an inhibitory ligand of the Notch signaling pathway that is predominantly localizes to the Golgi apparatus (PMID: 17664336) in normal condition. Normal tissue expression of DLL3 is highest in fetal brain, and DLL3 plays a key role in somitogenesis in the paraxial mesoderm (PMID: 26311731). It has been reported that DLL3 is expressed on the surface of tumor cells of small cell lung cancer (SCLC) and high-grade neuroendocrine carcinomas (LCNEC) and has emerged as a novel therapeutic target (PMID: 26311731; 28487384).