HURP Polyclonal antibody

• Featured Product
• KD/KO Validated

HURP Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12038-1-AP

Synonyms

DAP 5, Discs large homolog 7, DLG7, DLGAP5, Hepatoma up regulated protein, HURP, KIAA0008

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Validation Data Gallery

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  WB analysis of HEK-293 using 12038-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12038-1-AP (DLGAP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12038-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12038-1-AP (DLGAP5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 12038-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 12038-1-AP (HURP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

12038-1-AP targets HURP in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HURP fusion protein Ag2667
• Full Name: discs, large (Drosophila) homolog-associated protein 5
• Calculated Molecular Weight: 846 aa, 95 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC010658
• Gene Symbol: DLGAP5
• Gene ID (NCBI): 9787
• RRID: AB_10665363
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLGAP5, also named as DAP 5 or DLG7, is a kinetochore protein that stabilizes microtubules in vicinity of chromosomes. DLG7 controls spindle dynamics, promotes interkinetochore tension and efficient kinetochore capture. DGL7 is a part of Ran-dependent complex. Expression of DGL7 is found in cancer and stem cells. DLGAP5 contributes to hepatocellular carcinoma tumorigenesis by promoting cell proliferation (PMID: 24324629).

Publications

Species	Application	Title
human	IHC	J Transl Med; Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance; Authors - Kun Zheng
pig	IF	Cells; Mitotic Acetylation of Microtubules Promotes Centrosomal PLK1 Recruitment and Is Required to Maintain Bipolar Spindle Homeostasis.; Authors - Sylvia Fenosoa Rasamizafy
human	WB,IHC	Life Sci; Pan-cancer analysis and experiments with cell lines reveal that the slightly elevated expression of DLGAP5 is involved in clear cell renal cell carcinoma progression.; Authors - Yun Feng ; KD Validated
human	WB	Front Endocrinol (Lausanne); Whole Genome Transcriptomic Analysis of Ovary Granulosa Cells Revealed an Anti-Apoptosis Regulatory Gene DLGAP5 in Polycystic Ovary Syndrome.; Authors - Yan Deng
human	WB,IHC	Dis Markers; High Expression of DLGAP5 Indicates Poor Prognosis and Immunotherapy in Lung Adenocarcinoma and Promotes Proliferation through Regulation of the Cell Cycle; Authors - Xiaolong Tang ; KD Validated