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ARHI Polyclonal antibody

ARHI Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 10811-2-AP

Synonyms

ARHI, DIRAS3, GTP binding protein Di Ras3, NOEY2, RHOI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHI Polyclonal antibody (10811-2-AP)

    WB analysis using 10811-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10811-2-AP (ARHI antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ARHI Polyclonal antibody (10811-2-AP)

    IHC staining of human pancreas cancer using 10811-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10811-2-AP (ARHI antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ARHI Polyclonal antibody (10811-2-AP)

    IHC staining of human pancreas cancer using 10811-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10811-2-AP (ARHI antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10811-2-AP targets ARHI in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHI fusion protein Ag1123
Full Name DIRAS family, GTP-binding RAS-like 3
Calculated Molecular Weight 26 kDa
Observed Molecular Weight26-30 kDa
GenBank Accession NumberBC005362
Gene Symbol DIRAS3
Gene ID (NCBI) 9077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ARHI antibody 10811-2-APDownload protocol
IHC protocol for ARHI antibody 10811-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols