DIRAS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DIRAS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12634-1-AP

Synonyms

Di Ras1, DIRAS1, GBTS1, GTP binding protein Di Ras1, RIG

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Validation Data Gallery

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  WB analysis of human brain using 12634-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12634-1-AP (DIRAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12634-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12634-1-AP (DIRAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12634-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12634-1-AP (DIRAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12634-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12634-1-AP (DIRAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12634-1-AP

  IP result of anti-DIRAS1 (IP:12634-1-AP, 4ug; Detection:12634-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

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  IHC staining of human oesophagus using 12634-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 12634-1-AP (DIRAS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus using 12634-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 12634-1-AP (DIRAS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue, human placenta tissue, MCF-7 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human oesophagus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

12634-1-AP targets DIRAS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DIRAS1 fusion protein Ag3329
• Full Name: DIRAS family, GTP-binding RAS-like 1
• Calculated Molecular Weight: 198 aa, 22 kDa
• Observed Molecular Weight: 22-24 kDa
• GenBank Accession Number: BC030660
• Gene Symbol: DIRAS1
• Gene ID (NCBI): 148252
• RRID: AB_2292771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DIRAS1 belongs to the small guanosine triphosphatase (GTPase) Ras superfamily. DIRAS1 is frequently downregulated in human cancers due to DNA copy number loss, loss of heterozygosity (LOH), and hypermethylation of the promoter. The restoration of DIRAS1 has been reported to suppress tumor growth and metastasis in human neural tumors, esophageal cancer, colorectal cancer, and ovarian cancer.

Publications

Species	Application	Title
human	WB	Cancers (Basel); Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas.; Authors - Tanja Rothhammer-Hampl
human	WB,IHC	Mol Ther Nucleic Acids; RNAa and Vector-Mediated Overexpression of DIRAS1 Suppresses Tumor Growth and Migration in Renal Cell Carcinoma.; Authors - Xin Xu ; KD Validated
human	WB	J Biol Chem; The Tumor Suppressive Small GTPase DiRas1 Binds the Non-canonical Guanine Nucleotide Exchange Factor SmgGDS and Antagonizes SmgGDS Interactions with Oncogenic Small GTPases.; Authors - Carmen Bergom
rat	WB,IHC	Int J Environ Res Public Health; Effects of In Utero Exposure to Di-n-Butyl Phthalate on Testicular Development in Rat.; Authors - Tan Ma