DIO2 Polyclonal antibody

DIO2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 26513-1-AP

Synonyms

5DII, D2, DIO2, DIOII, ITDI2, SelY, TXDI2, Type 2 DI, Type II 5 deiodinase

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 26513-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 26513-1-AP (DIO2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26513-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26513-1-AP (DIO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 26513-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26513-1-AP (DIO2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 26513-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26513-1-AP (DIO2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, MCF-7 cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 26513-1-AP is DIO2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DIO2 fusion protein Ag24119
• Full Name: deiodinase, iodothyronine, type II
• Calculated Molecular Weight: 273 aa, 31 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC074882
• Gene Symbol: DIO2
• Gene ID (NCBI): 1734
• RRID: AB_2880538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type II iodothyronine deiodinase(DIO2), belongs to the iodothyronine deiodinase family, with two isoform of 30 and 34 kDa. DIO2 can activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). DIO2 is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Tumor Cell "Slimming" Regulates Tumor Progression through PLCL1/UCP1-Mediated Lipid Browning.; Authors - Zhiyong Xiong
human	WB	Nat Chem Biol; Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability.; Authors - Zhipeng Li
mouse	WB	Diabetes; Id1 Promotes Obesity by Suppressing Brown Adipose Thermogenesis and White Adipose Browning.; Authors - Mallikarjun Patil
human	IHC,WB	Clin Transl Med; NNT-induced tumor cell "slimming" reverses the pro-carcinogenesis effect of HIF2a in tumors.; Authors - Zhiyong Xiong
mouse	WB	FASEB J; The tumor secretory factor ZAG promotes white adipose tissue browning and energy wasting.; Authors - Sawsan Elattar
mouse	WB	Ecotoxicol Environ Saf; Exposure to melamine cyanuric acid in adult mice induced thyroid dysfunction and circadian rhythm disorder.; Authors - Sijie Wang