DIO1 Polyclonal antibody

DIO1 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 11790-1-AP

Synonyms

5DI, DIO1, DIOI, ITDI1, TXDI1, Type 1 DI, Type I 5 deiodinase

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Validation Data Gallery

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  WB analysis of mouse lung using 11790-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11790-1-AP (DIO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 11790-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11790-1-AP (DIO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 11790-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 11790-1-AP (DIO1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11790-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11790-1-AP (DIO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11790-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11790-1-AP (DIO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11790-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11790-1-AP (DIO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11790-1-AP (DIO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11790-1-AP (DIO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11790-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11790-1-AP (DIO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11790-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11790-1-AP (DIO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11790-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11790-1-AP (DIO1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11790-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11790-1-AP (DIO1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 11790-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using DIO1 antibody (11790-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11790-1-AP

  1X10^6 HeLa cells were stained with 0.20ug DIO1 antibody (11790-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: mouse lung tissue, HeLa cells, mouse ovary tissue, rat lung tissue, U-937 cells, A549 cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

11790-1-AP targets DIO1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DIO1 fusion protein Ag2410
• Full Name: deiodinase, iodothyronine, type I
• Calculated Molecular Weight: 249 aa, 29 kDa
• Observed Molecular Weight: 29 kDa, 13 kDa
• GenBank Accession Number: BC017955
• Gene Symbol: DIO1
• Gene ID (NCBI): 1733
• RRID: AB_10949197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DIO1(Type I iodothyronine deiodinase) is also named as ITDI1, TXDI1,5DI,DIOI and belongs to the iodothyronine deiodinase family.It is a membrane selenoprotein that catalyzes the deiodination of L-thyroxine to the biologically active thyroid hormone 3,3′,5-triiodothyronine.DIO1 is located in liver, kidney, and thyroid,which has both ORD and IRD activities(PMID: 9389495). It has some isoforms produced by alternative splicing with the MW of 29 kDa, 21 kDa, 13 kDa, 23 kDa, 19 kDa, 4 kDa, 13 kDa, 9 kDa and 10 kDa.

Publications

Species	Application	Title
mouse	IF	Cell Rep; Environmental Enrichment Induces Pericyte and IgA-Dependent Wound Repair and Lifespan Extension in a Colon Tumor Model.; Authors - Benjamin D Bice
rat	WB,IHC	Chemosphere; Rno-miR-224-5p contributes to 2,2',4,4'-tetrabromodiphenyl ether-induced low triiodothyronine in rats by targeting deiodinases.; Authors - Chao Wang
human, mouse	WB, IHC	J Biol Chem; Astrocyte Elevated Gene-1 (AEG-1) Contributes to Nonthyroidal Illness Syndrome (NTIS) Associated with Hepatocellular Carcinoma (HCC).; Authors - Jyoti Srivastava
mouse	WB	Sci Rep; Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets.; Authors - B Chellan
rat	WB	Front Physiol; Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding.; Authors - Paula B M De Andrade
mouse	WB	Life Sci; Role of diets and exercise in ameliorating obesity-related hepatic steatosis: Insights at the microRNA-dependent thyroid hormone synthesis and action.; Authors - Shu-Fang Xia