DHRS7C Polyclonal antibody

DHRS7C Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26020-1-AP

Synonyms

DHRS7C, SDR32C2

---

Validation Data Gallery

• 

  WB analysis using 26020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26020-1-AP (DHRS7C antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 26020-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26020-1-AP (DHRS7C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26020-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26020-1-AP (DHRS7C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: rat skeletal muscle tissue, mouse skeletal muscle tissue
• Positive IHC detected in: mouse skeletal muscle tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26020-1-AP targets DHRS7C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHRS7C fusion protein Ag23392
• Full Name: dehydrogenase/reductase (SDR family) member 7C
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC147025
• Gene Symbol: DHRS7C
• Gene ID (NCBI): 201140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.