DGKE Polyclonal antibody

DGKE Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11900-1-AP

Synonyms

DAG kinase epsilon, DAGK5, DAGK6, DGK, DGK epsilon, DGKE, Diacylglycerol kinase epsilon, Diglyceride kinase epsilon

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Validation Data Gallery

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  WB analysis using 11900-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11900-1-AP (DGKE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11900-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11900-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11900-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 11900-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DGKE antibody (11900-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11900-1-AP targets DGKE in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DGKE fusion protein Ag2512
• Full Name: diacylglycerol kinase, epsilon 64kDa
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 60-64 kDa
• GenBank Accession Number: BC022297
• Gene Symbol: DGKE
• Gene ID (NCBI): 8526
• RRID: AB_2091145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DGKE(Diacylglycerol kinase epsilon) is also named as DAGK5 and belongs to the eukaryotic diacylglycerol kinase family.It may be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction(PMID:16689942).When expressed in mammalian cells, DGK-epsilon showed specificity for arachidonyl-containing diacylglycerol.

Publications

Species	Application	Title
mouse	IHC	Sci Rep; microRNA regulatory circuits in a mouse model of inherited retinal degeneration.; Authors - Arpad Palfi
human	WB	Obesity (Silver Spring); Rutaecarpin reduces lipids by DGKθ-dependent activation of PPARα; Authors - Di Wu
mouse	WB,IF,IP	Biochim Biophys Acta Mol Cell Biol Lipids; Interaction of ROMK2 channel with lipid kinases DGKE and AGK: Potential channel activation by localized anionic lipid synthesis; Authors - Milena Krajewska