CoraLite® Plus 488-conjugated DGKA Polyclonal antibody

• Featured Product
• KD/KO Validated

DGKA Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11547

Synonyms

80 kDa diacylglycerol kinase, DAG kinase alpha, DAGK, DAGK1, DGK alpha, DGKA, Diacylglycerol kinase alpha, Diglyceride kinase alpha

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Validation Data Gallery

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  IF Staining of Jurkat using CL488-11547

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 DGKA antibody (CL488-11547) at dilution of 1:200.

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  FC experiment of HeLa using CL488-11547

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human DGKA (CL488-11547) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: Jurkat cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11547 targets DGKA in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DGKA fusion protein Ag2075
• Full Name: diacylglycerol kinase, alpha 80kDa
• Calculated Molecular Weight: 735 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC023523
• Gene Symbol: DGKA
• Gene ID (NCBI): 1606
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.