DEK Recombinant antibody

DEK Recombinant Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

230065H2

Cat no : 82880-1-RR

Synonyms

D6S231E, DEK, DEK oncogene, Protein DEK

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Validation Data Gallery

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  WB analysis using 82880-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82880-1-RR (DEK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 82880-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DEK antibody (82880-1-RR, Clone: 230065H2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

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  IF Staining of HeLa using 82880-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DEK antibody (82880-1-RR, Clone: 230065H2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells
• Positive IF detected in: U2OS cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82880-1-RR targets DEK in WB, IF, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DEK fusion protein Ag8225
• Full Name: DEK oncogene
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC035259
• Gene Symbol: DEK
• Gene ID (NCBI): 7913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.