DEAF1 Polyclonal antibody

DEAF1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18323-1-AP

Synonyms

DEAF1, NUDR, SPN, Suppressin, ZMYND5

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Validation Data Gallery

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  WB analysis using 18323-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18323-1-AP (DEAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 18323-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18323-1-AP (DEAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 18323-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of Jurkat using 18323-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DEAF1 antibody (18323-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, BxPC-3 cells, Jurkat cells, SH-SY5Y cells
• Positive IHC detected in: human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human lung tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18323-1-AP targets DEAF1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DEAF1 fusion protein Ag13173
• Full Name: deformed epidermal autoregulatory factor 1 (Drosophila)
• Calculated Molecular Weight: 565 aa, 59 kDa
• Observed Molecular Weight: 60-63 kDa, 72 kDa
• GenBank Accession Number: BC053322
• Gene Symbol: DEAF1
• Gene ID (NCBI): 10522
• RRID: AB_2261595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.