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CoraLite®594-conjugated DDX5 Monoclonal antibody

DDX5 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D3D2

Cat no : CL594-67025

Synonyms

DDX5, DEAD box protein 5, DKFZp434E109, DKFZp686J01190, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated DDX5 Monoclonal antibody (CL594-67025)

    IF Staining of HepG2 using CL594-67025

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated DDX5 antibody (CL594-67025, Clone: 2D3D2 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated DDX5 Monoclonal antibody (CL594-67025)

    FC experiment of HeLa using CL594-67025

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human DDX5 (CL594-67025, Clone:2D3D2) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67025 targets DDX5 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DDX5 fusion protein Ag24821
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Observed Molecular Weight 69 kDa
GenBank Accession NumberNM_004396
Gene Symbol DDX5
Gene ID (NCBI) 1655
RRIDAB_2920073
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for CL594 DDX5 antibody CL594-67025Download protocol
FC protocol for CL594 DDX5 antibody CL594-67025Download protocol
Standard Protocols
Click here to view our Standard Protocols