DDX5,p68 Polyclonal antibody

• Featured Product
• KD/KO Validated

DDX5,p68 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 10804-1-AP

Synonyms

DDX5, DDX5,p68, DEAD box protein 5, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 10804-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5,p68 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10804-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5,p68 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10804-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5,p68 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10804-1-AP (DDX5,p68 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10804-1-AP (DDX5,p68 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, A431 cells, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

10804-1-AP targets DDX5,p68 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX5,p68 fusion protein Ag1237
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 65-68 kDa
• GenBank Accession Number: BC016027
• Gene Symbol: DDX5
• Gene ID (NCBI): 1655
• RRID: AB_2292757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation. For the reason that DDX5 can be phosphorylated and dephosphorylated at several sites, at some cases the molecular weight of DDX5 is less than 68kD, approximately 65kd

Publications

Species	Application	Title
human,mouse	WB,IHC	Drug Des Devel Ther; Vancomycin Induced Ferroptosis in Renal Injury Through the Inactivation of Recombinant Glutathione Peroxidase 4 and the Accumulation of Peroxides; Authors - Xuedong Yin
human	IP	Cell Mol Life Sci; Ribosomal protein L24 mediates mammalian microRNA processing in an evolutionarily conserved manner; Authors - Yonat Tzur
human	WB	RNA Biol; Modulation of PDCD1 exon 3 splicing.; Authors - Junjie Sun ; KD Validated
human	WB,IF,IP	Microbiol Spectr; DDX5/METTL3-METTL14/YTHDF2 Axis Regulates Replication of Influenza A Virus.; Authors - Lingcai Zhao ; KD Validated
mouse	WB	Gene; Intron retention as a new pre-symptomatic marker of aging and its recovery to the normal state by a traditional Japanese multi-herbal medicine.; Authors - Norihiro Okada
mouse	WB	Data Brief; Data of RNA-seq transcriptomes of liver, bone, heart, kidney and blood in klotho mice at a pre-symptomatic state and the effect of a traditional Japanese multi-herbal medicine, juzentaihoto.; Authors - Norihiro Okada