DDX10 Polyclonal antibody

• Featured Product
• KD/KO Validated

DDX10 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 17857-1-AP

Synonyms

DDX10, DEAD box protein 10, HRH J8

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Validation Data Gallery

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  WB analysis of SMMC-7721 using 17857-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17857-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 17857-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse testis using 17857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17857-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: SMMC-7721 cells, HeLa cells
• Positive IHC detected in: mouse testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

17857-1-AP targets DDX10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX10 fusion protein Ag12317
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
• Calculated Molecular Weight: 875 aa, 101 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC091521
• Gene Symbol: DDX10
• Gene ID (NCBI): 1662
• RRID: AB_2878454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX10, DEAD-box helicase 10, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Fusion of this gene and the nucleoporin gene NUP98 is found in leukemogenesis (PMID:20339440).

Publications

Species	Application	Title
human	WB,IHC	Cancer Cell Int; DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35.; Authors - Xin Zhou ; KD Validated
human	WB	Biochem Biophys Res Commun; Epigenetic down-regulated DDX10 promotes cell proliferation through Akt/NF-κB pathway in ovarian cancer.; Authors - Muhuizi Gai ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wael (Verified Customer) (05-24-2023)

We detected the DDX10 relative abundance in both the nuclear and cytoplasmic fractions

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293

DDX10 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:17857-1-AP)