CoraLite®594-conjugated DDX1 Monoclonal antibody

DDX1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G10G4

Cat no : CL594-67991

Synonyms

DBP RB, DDX1, DEAD box protein 1, UKVH5d

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL594-67991

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 DDX1 antibody (CL594-67991, Clone: 1G10G4 ) at dilution of 1:400.

---

Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67991 targets DDX1 in IF applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DDX1 fusion protein Ag16774
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
• Calculated Molecular Weight: 740 aa, 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC012132
• Gene Symbol: DDX1
• Gene ID (NCBI): 1653
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.